Proteomic changes in Actinidia chinensis shoot during systemic infection with a pandemic Pseudomonas syringae pv. actinidiae strain.
Identifieur interne : 000209 ( Main/Exploration ); précédent : 000208; suivant : 000210Proteomic changes in Actinidia chinensis shoot during systemic infection with a pandemic Pseudomonas syringae pv. actinidiae strain.
Auteurs : Milena Petriccione [Italie] ; Ilaria Di Cecco ; Simona Arena ; Andrea Scaloni ; Marco ScortichiniSource :
- Journal of proteomics [ 1876-7737 ] ; 2013.
Descripteurs français
- KwdFr :
- Actinidia (microbiologie), Actinidia (métabolisme), Interactions hôte-pathogène (physiologie), Maladies des plantes (MeSH), Pousses de plante (microbiologie), Pousses de plante (métabolisme), Protéines végétales (métabolisme), Protéome (métabolisme), Protéomique (MeSH), Pseudomonas syringae (physiologie).
- MESH :
- microbiologie : Actinidia, Pousses de plante.
- métabolisme : Actinidia, Pousses de plante, Protéines végétales, Protéome.
- physiologie : Interactions hôte-pathogène, Pseudomonas syringae.
- Maladies des plantes, Protéomique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Plant Proteins, Proteome.
- metabolism : Actinidia, Plant Shoots.
- microbiology : Actinidia, Plant Shoots.
- physiology : Host-Pathogen Interactions, Pseudomonas syringae.
- Plant Diseases, Proteomics.
Abstract
A pandemic, very aggressive population of Pseudomonas syringae pv. actinidiae is currently causing severe economic losses to kiwifruit crops worldwide. Upon leaf attack, this Gram-negative bacterium systemically reaches the plant shoot in a week period. In this study, combined 2-DE and nanoLC-ESI-LIT-MS/MS procedures were used to describe major proteomic changes in Actinidia chinensis shoot following bacterial inoculation in host leaf. A total of 117 differentially represented protein spots were identified in infected and control shoots. Protein species associated with plant defence, including type-members of the plant basal defence, pathogenesis, oxidative stress and heat shock, or with transport and signalling events, were the most represented category of induced components. Proteins involved in carbohydrate metabolism and photosynthesis were also augmented upon infection. In parallel, a bacterial outer membrane polypeptide component was identified in shoot tissues, whose homologues were already linked to bacterial virulence in other eukaryotes. Semiquantitative RT-PCR analysis confirmed expression data for all selected plant gene products. All these data suggest a general reprogramming of shoot metabolism following pathogen systemic infection, highlighting organ-specific differences within the context of a general similarity with respect to other pathosystems. In addition to present preliminary information on the molecular mechanisms regulating this specific plant-microbe interaction, our results will foster future proteomic studies aimed at characterizing the very early events of host colonization, thus promoting the development of novel bioassays for pathogen detection in kiwifruit material.
DOI: 10.1016/j.jprot.2012.10.014
PubMed: 23099348
Affiliations:
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Arena</name></author><author><name sortKey="Scaloni, Andrea" sort="Scaloni, Andrea" uniqKey="Scaloni A" first="Andrea" last="Scaloni">Andrea Scaloni</name></author><author><name sortKey="Scortichini, Marco" sort="Scortichini, Marco" uniqKey="Scortichini M" first="Marco" last="Scortichini">Marco Scortichini</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23099348</idno><idno type="pmid">23099348</idno><idno type="doi">10.1016/j.jprot.2012.10.014</idno><idno type="wicri:Area/Main/Corpus">000223</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000223</idno><idno type="wicri:Area/Main/Curation">000223</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000223</idno><idno type="wicri:Area/Main/Exploration">000223</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Proteomic changes in Actinidia chinensis 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(métabolisme)</term><term>Interactions hôte-pathogène (physiologie)</term><term>Maladies des plantes (MeSH)</term><term>Pousses de plante (microbiologie)</term><term>Pousses de plante (métabolisme)</term><term>Protéines végétales (métabolisme)</term><term>Protéome (métabolisme)</term><term>Protéomique (MeSH)</term><term>Pseudomonas syringae (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term><term>Proteome</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Actinidia</term><term>Plant Shoots</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Actinidia</term><term>Pousses de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Actinidia</term><term>Plant Shoots</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Actinidia</term><term>Pousses de plante</term><term>Protéines végétales</term><term>Protéome</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Interactions hôte-pathogène</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Host-Pathogen Interactions</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Plant Diseases</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Maladies des plantes</term><term>Protéomique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A pandemic, very aggressive population of Pseudomonas syringae pv. actinidiae is currently causing severe economic losses to kiwifruit crops worldwide. Upon leaf attack, this Gram-negative bacterium systemically reaches the plant shoot in a week period. In this study, combined 2-DE and nanoLC-ESI-LIT-MS/MS procedures were used to describe major proteomic changes in Actinidia chinensis shoot following bacterial inoculation in host leaf. A total of 117 differentially represented protein spots were identified in infected and control shoots. Protein species associated with plant defence, including type-members of the plant basal defence, pathogenesis, oxidative stress and heat shock, or with transport and signalling events, were the most represented category of induced components. Proteins involved in carbohydrate metabolism and photosynthesis were also augmented upon infection. In parallel, a bacterial outer membrane polypeptide component was identified in shoot tissues, whose homologues were already linked to bacterial virulence in other eukaryotes. Semiquantitative RT-PCR analysis confirmed expression data for all selected plant gene products. All these data suggest a general reprogramming of shoot metabolism following pathogen systemic infection, highlighting organ-specific differences within the context of a general similarity with respect to other pathosystems. In addition to present preliminary information on the molecular mechanisms regulating this specific plant-microbe interaction, our results will foster future proteomic studies aimed at characterizing the very early events of host colonization, thus promoting the development of novel bioassays for pathogen detection in kiwifruit material.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23099348</PMID><DateCompleted><Year>2013</Year><Month>07</Month><Day>04</Day></DateCompleted><DateRevised><Year>2016</Year><Month>05</Month><Day>18</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1876-7737</ISSN><JournalIssue CitedMedium="Internet"><Volume>78</Volume><PubDate><Year>2013</Year><Month>Jan</Month><Day>14</Day></PubDate></JournalIssue><Title>Journal of proteomics</Title><ISOAbbreviation>J Proteomics</ISOAbbreviation></Journal><ArticleTitle>Proteomic changes in Actinidia chinensis shoot during systemic infection with a pandemic Pseudomonas syringae pv. actinidiae strain.</ArticleTitle><Pagination><MedlinePgn>461-76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jprot.2012.10.014</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1874-3919(12)00709-9</ELocationID><Abstract><AbstractText>A pandemic, very aggressive population of Pseudomonas syringae pv. actinidiae is currently causing severe economic losses to kiwifruit crops worldwide. Upon leaf attack, this Gram-negative bacterium systemically reaches the plant shoot in a week period. In this study, combined 2-DE and nanoLC-ESI-LIT-MS/MS procedures were used to describe major proteomic changes in Actinidia chinensis shoot following bacterial inoculation in host leaf. A total of 117 differentially represented protein spots were identified in infected and control shoots. Protein species associated with plant defence, including type-members of the plant basal defence, pathogenesis, oxidative stress and heat shock, or with transport and signalling events, were the most represented category of induced components. Proteins involved in carbohydrate metabolism and photosynthesis were also augmented upon infection. In parallel, a bacterial outer membrane polypeptide component was identified in shoot tissues, whose homologues were already linked to bacterial virulence in other eukaryotes. Semiquantitative RT-PCR analysis confirmed expression data for all selected plant gene products. All these data suggest a general reprogramming of shoot metabolism following pathogen systemic infection, highlighting organ-specific differences within the context of a general similarity with respect to other pathosystems. In addition to present preliminary information on the molecular mechanisms regulating this specific plant-microbe interaction, our results will foster future proteomic studies aimed at characterizing the very early events of host colonization, thus promoting the development of novel bioassays for pathogen detection in kiwifruit material.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Petriccione</LastName><ForeName>Milena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research Unit for Fruit Trees, Agricultural Research Council (CRA), 81100 Caserta, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Di Cecco</LastName><ForeName>Ilaria</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Arena</LastName><ForeName>Simona</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Scaloni</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Scortichini</LastName><ForeName>Marco</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Proteomics</MedlineTA><NlmUniqueID>101475056</NlmUniqueID><ISSNLinking>1874-3919</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020543">Proteome</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029042" MajorTopicYN="Y">Actinidia</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="Y">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="Y">Plant Shoots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020543" MajorTopicYN="N">Proteome</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044224" MajorTopicYN="N">Pseudomonas syringae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>10</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>10</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>10</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>7</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23099348</ArticleId><ArticleId IdType="pii">S1874-3919(12)00709-9</ArticleId><ArticleId IdType="doi">10.1016/j.jprot.2012.10.014</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Arena, Simona" sort="Arena, Simona" uniqKey="Arena S" first="Simona" last="Arena">Simona Arena</name><name sortKey="Di Cecco, Ilaria" sort="Di Cecco, Ilaria" uniqKey="Di Cecco I" first="Ilaria" last="Di Cecco">Ilaria Di Cecco</name><name sortKey="Scaloni, Andrea" sort="Scaloni, Andrea" uniqKey="Scaloni A" first="Andrea" last="Scaloni">Andrea Scaloni</name><name sortKey="Scortichini, Marco" sort="Scortichini, Marco" uniqKey="Scortichini M" first="Marco" last="Scortichini">Marco Scortichini</name></noCountry><country name="Italie"><noRegion><name sortKey="Petriccione, Milena" sort="Petriccione, Milena" uniqKey="Petriccione M" first="Milena" last="Petriccione">Milena Petriccione</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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